RNA Interference-Based Pesticides and Antiviral Agents: Microbial Overproduction Systems for Double-Stranded RNA for Applications in Agriculture and Aquaculture

RNA interference (RNAi)-based pesticides are pest control agents that use RNAi mechanisms as the basis of their action. They regarded environmentally friendly and a promising alternative to conventional chemical pesticides. The effective substance in RNAi-based is double-stranded (dsRNA) designed match nucleotide sequence target essential gene concern. When taken up by pest, this exerts an effect inhibits some vital biochemical/biological process pest. dsRNA products also expected be applied for viral diseases aquaculture RNAi, especially shrimp farming. A critical issue practical application production must low-cost. Here, we review recent methods microbial dsRNAs using representative microorganisms (Escherichia coli, Pseudomonas syringae, Corynebacterium glutamicum, Chlamydomonas reinhardtii, others) host strains. characteristics each system discussed.